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Two types of medications for ulcers and gastric reflux proton-pump inhibitors, such as omeprazole (Prilosec) and lansoprazole (Prevacid), and H2 blockers like cimetidine (Tagamet), famotidine (Pepcid), and ranitidine (Zantac) inhibit the release of stomach acid necessary to free dietary vitamin B12

Pandey KB, Rizvi SI

Vigorous shaking creates foam, which means air is being forced into the solution, and the mechanical stress of bubble formation can denature the peptide

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In DPPH method, 50 L of solutions prepared same as in UV-VIS analysis was taken into wells of 96-well test microplate with 250 L of a 0.36 mM methanol solution of DPPH